Guinea Pig IgM Antibody

SKU: orb346138

Description

Guinea Pig IgM Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA, SDS-PAGE
• Application Notes:
  Guinea Pig IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Guinea Pig
• Biological Origin: Guinea Pig
• Isotype: IgM
• Purity: Guinea Pig Igm whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Guinea Pig Igm whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum and anti-Guinea Pig IgM (µ chain specific). No reaction was observed against anti-Guinea Pig IgG F(c). Some light chain cross reactivity will occur with anti-Guinea Pig IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. Guinea Pig Igm whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 0.88 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Guinea Pig immunoglobulin M; GP IgM

SDS-Page of Guinea Pig IgM Whole Molecule. Lane 1: Guinea Pig IgM, Non-Reduced. Lane 2: Guinea Pig IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.