Guinea Pig IgG F(ab')2 Antibody Biotin Conjugated

• Catalog Number: orb347092
• Category: Antibodies
• Description: Guinea Pig IgG F(ab')2 antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Guinea pig
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c).
• Immunogen: Guinea Pig IgG F(ab')2 fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit Anti-Guinea Pig IgG F(ab')2 Biotin Conjugat
• Note: For research use only