GSTK1 Antibody

SKU: orb344488

Description

GSTK1 antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000 - 1:200,000, WB: 1:500 - 1:1000
• Reactivity: Human
• Application Notes:
  Anti-GSTK1 antibody is tested by in ELISA, SDS-Page, and Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 16A11.E4.E3.E7
• Immunogen: This Protein A purified antibody was prepared by repeated immunizations in mice with K1 Protein.
• Target: GSTK1
• Purity: This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above. Reacts specifically with K1 protein. Cross reactivity from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-GSTK1 antibody, mouse anti-GST-K1 antibody, GST, Glutathione S-Transferases, K1 antibody, GSTK1

SDS-PAGE of Mouse anti-GSTK1 Monoclonal Antibody. Lane 1: Reduced Mouse anti-GSTK1 Monoclonal Antibody. Lane M: 3 µl OPAL Pre-stained Marker. Lane 2: Non-reduced Mouse anti-GSTK1 Monoclonal Antibody. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.

Western Blot of Mouse anti-GSTK1 Monoclonal Antibody. Lane 1: Recombinant GSTK1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTK1 Monoclonal Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP Mouse Secondary Antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTK1.

Quick Database Links

Gene Symbol

GSTK1

UniProt

Q9Y2Q3