GST Antibody

SKU: orb344221

Description

GST antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:200 - 1:1000, WB: 1:1000 - 1:5000
• Application Notes:
  Anti-GST antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
• Purity: Anti-GST is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store GST antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-GST antibody, GST, Glutathione-S-Transferase

Biorbyt Goat anti GST antibody was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For blot on the left, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti GST (1:5K in orb348637, ON 4 C). Primary antibody was detected with Biorbyt Dylight 649 conjugated Donkey anti Goat (1:10K 1.5 hr RT in orb348637). Blot on right shows a repeat western blot with the same samples (~1 ug per lane, reduced (R) and non-reduced (NR) probed 1:1000 dilution of primary antibody and detection using Dylight 549 conjugated Donkey anti goat (1:10K 1.5 hr RT in orb348637).