GRM7 Antibody

• Catalog Number: orb621260
• Category: Antibodies
• Description: GRM7 Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Clone Number: D3-G8
• Tested applications: FC, ICC, WB
• Reactivity: Human
• Immunogen: Recombinant protein
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2,000, ICC: 1:50-1:200, FC: 1:50-1:100
• Form/Appearance: Liquid
• Purity: ProA affinity purified
• Conjugation: Unconjugated
• MW: 102 kDa
• UniProt ID: Q14831
• Storage: Store at -20˚C
• Buffer/Preservatives: Formulation 1*TBS (pH7.4), 1%rAlbumin, 40%Glycerol. Preservative: 0.05% Sodium Azide
• Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GRM7 on human GRM7 recombinant protein using anti-GRM7 antibody at 1/1000 dilution

Western blot analysis of GRM7 on HEK293 (1) and GRM7-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM7 antibody at 1/1000 dilution

ICC staining GRM7 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

Flow cytometric analysis of SH-SY5Y cells with GRM7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red)