GRK1 Antibody

SKU: orb1256972

Description

GRK1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
• Target: GRK1
• Molecular Weight: Observed: 65kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rhodopsin kinase, RK, G protein-coupled receptor kinase 1, GRK1, RHOK

Western blot analysis of extracts of Y79 cells, using GRK1 antibody (orb1256972). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

GRK1

UniProt

Q15835