GRK1 Antibody

• Catalog Number: orb1256972
• Category: Antibodies
• Description: GRK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 65kDa
• Target: GRK1
• UniProt ID: Q15835
• Storage: Store at 4°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, pH 7.3.
• Alternative names: Rhodopsin kinase, RK, G protein-coupled receptor k
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Y79 cells, using GRK1 antibody (orb1256972). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.