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Catalog Number | orb1256972 |
---|---|
Category | Antibodies |
Description | GRK1 Antibody |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 65kDa |
Target | GRK1 |
UniProt ID | Q15835 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Rhodopsin kinase, RK, G protein-coupled receptor k Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of Y79 cells, using GRK1 antibody (orb1256972). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of rat retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse retina using GRK1 Polyclonal Antibody (orb1256972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |