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Description
Images & Validation
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Key Properties
−| Source | Baculovirus |
|---|---|
| Biological Activity | Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac - IEPD - pNA substrate at 25˚C, is greater than 750 nM/min per μg of enzyme. |
| Target | Gzmb |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS |
Storage & Handling
−| Storage | The lyophilized Granzyme B recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Granzyme B is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Granzyme B recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, CTLA-1
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_038570.1 |
|---|
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Granzyme B Recombinant Protein (orb1230964)
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