GPX7 Antibody

• Catalog Number: orb1247421
• Category: Antibodies
• Description: GPX7 Antibody
• Target: GPX7
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: CNQFGQQEPDSNK
• UniProt ID: Q96SL4
• Tested applications: EIA, ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20kDa band observed in Human Prostate lysates (calculated MW of 21.0kDa according to NP_056511.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPX7, glutathione peroxidase 7 [Homo sapiens] , HG
• Note: For research use only
• NCBI: NP_056511.2

orb1247421 (0.3 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247421 (5 ug/ml) as the reporter antibody.