GPX7 Antibody

SKU: orb1247421

Description

GPX7 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: EIA, ELISA, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20kDa band observed in Human Prostate lysates (calculated MW of 21.0kDa according to NP_056511.2). Recommended concentration: 0.3-1µg/mL. An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. Recommended reporter concentration: 5-10µg/mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CNQFGQQEPDSNK
• Target: GPX7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPX7, glutathione peroxidase 7 [Homo sapiens] , HGNC:4559, CL683, FLJ14777, GPX6, NPGPx, non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase

orb1247421 (0.3 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247421 (5 ug/ml) as the reporter antibody.

Quick Database Links

Gene Symbol

GPX7

UniProt

Q96SL4

RefSeq (Protein):NP_056511.2