GP2 antibody

SKU: orb2276384

Description

GP2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity. Negative Control: Colon: GP2 staining should be completely absent in glandular and stromal cells. Cellular Localization: Cytoplasmic and cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone No.: MSVA-475M
• Immunogen: Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glycoprotein 2 (zymogen granule membrane); GP2; Pancreatic zymogen granule membrane associated protein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP77

A strong GP2 staining is seen in acinar cells of the pancreas. The staining is cytoplasmic and membranous and shows a distinct apical predominance.

Pancreas Acinar cell carcinoma showing strong GP 2 immunostaining of all tumor cells.

Rectum mucosa GP2 immunostaining is completely lacking in rectal mucosa.

Quick Database Links

UniProt

P55259