Golden Syrian Hamster IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb347127
• Category: Antibodies
• Description: Golden Syrian Hamster IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Golden Hamster
• Form/Appearance: Lyophilized
• Concentration: 1.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Golden Syrian Hamster IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
• Immunogen: Hamster IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Golden Syrian Hamster IgG Biotin Conjugation has been tested by ELISA and is assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Golden Syrian Hamster IgG.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Golden Syrian Hamster IgG (H&L) Antibody, Rabbit-a
• Note: For research use only

ELISA Results of Purified Rabbit Anti-Golden Syrian Hamster IgG Antibody Biotin Conjugated tested against purified Golden Syrian Hamster IgG Biotin. Each well was coated in duplicate with 1.0 µg of Golden Syrian Hamster IgG (p/n orb2652759). The working dilution is 1:57000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).