Golden Syrian Hamster IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb347113
• Category: Antibodies
• Description: Golden Syrian Hamster IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Golden Hamster
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. Diminished reactivity will occur against Armenian Hamster IgG.
• Immunogen: Anti-Golden Syrian Hamster IgG whole molecule was produced by repeated immunization with Golden Syrian Hamster IgG whole molecule in goat.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:7,000 - 1:35,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application notes: Anti-Golden Syrian Hamster IgG peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Golden Syrian Hamster Antibody, Gt-a-Golden Syrian
• Note: For research use only

ELISA results of purified Polyclonal Goat Anti-Golden Syrian Hamster IgG Antibody Peroxidase Conjugated tested against Hamster IgG. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).