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SIDT1 Antibody

SKU: orb19784

Description

Goat polyclonal antibody to SIDT1

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:32000, WB: 0.5 μg/ml
Predicted ReactivityHuman
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 50kDa band in Human Brain (Cerebellum) and Human Breast lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.8kDa according to NP_060169.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetSIDT1
Protein SequenceQEKENQTWNLQRKKN
Molecular Weight93.8
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti SIDT1 antibody, anti SID1 transmembrane family, member 1 antibody, anti B830021E24Rik antibody, anti FLJ20174 antibody, anti SID-1 antibody, anti SID1 antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SIDT1 Antibody (orb19784)

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Available Sizes

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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