SEPT6 Antibody

• Catalog Number: orb20255
• Category: Antibodies
• Description: Goat polyclonal antibody to 41888
• Target: SEPT6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DEVNAFKQRKTA
• RRID: AB_10750624
• MW: 48.9; 49.7; 49.2
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SEPT6 antibody, anti septin 6 antibody, anti
• Note: For research use only
• Entrez: 23157

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Primary incubation 1 hour at room temperature. Image A: Human Testes lysate at primary Ab concentration 1 ug/ml, Image B: Human Duodenum lysate at primary Ab concentration 0.1 ug/ml, Image C: Human Kidney lysate at primary Ab concentration 0.5 ug/ml, Image D: Human Tonsil lysate at primary Ab concentration 0.3 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Images A, B, C: Daudi, Jurkat, MOLT-4 cell lysates at primary Ab concentration 0.1 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

3.8 µg/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).