SEPT6 Goat Polyclonal Antibody

SKU: orb20255

Description

This product is a goat polyclonal antibody targeting SEPT6, supplied unconjugated at 0.5 mg/ml. Validated for multiple applications including ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and Western blot, it exhibits reactivity with human samples and shows predicted reactivity with bovine and canine species.

Research Area

Stem Cell & Developmental Biology; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine

Key Properties

• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.
• Target: SEPT6
• Protein Sequence: DEVNAFKQRKTA
• Molecular Weight: 48.9 kDa; 49.7 kDa; 49.2 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SEPT6 antibody, anti septin 6 antibody, anti KIAA0128 antibody, anti MGC16619 antibody, anti MGC20339 antibody, anti SEP2 antibody, anti SEPT2 antibody, anti septin 2 antibody

Quick Database Links

Gene Symbol

SEPT6

NCBI Gene ID

23157