SEPT6 Goat Polyclonal Antibody

SKU: orb20255

Description

This product is a goat polyclonal antibody targeting SEPT6, supplied unconjugated at 0.5 mg/ml. Validated for multiple applications including ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and Western blot, it exhibits reactivity with human samples and shows predicted reactivity with bovine and canine species.

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.
• Target: SEPT6
• Protein Sequence: DEVNAFKQRKTA
• Molecular Weight: 48.9 kDa; 49.7 kDa; 49.2 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SEPT6 antibody, anti septin 6 antibody, anti KIAA0128 antibody, anti MGC16619 antibody, anti MGC20339 antibody, anti SEP2 antibody, anti SEPT2 antibody, anti septin 2 antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Primary incubation 1 hour at room temperature. Image A: Human Testes lysate at primary Ab concentration 1 ug/ml, Image B: Human Duodenum lysate at primary Ab concentration 0.1 ug/ml, Image C: Human Kidney lysate at primary Ab concentration 0.5 ug/ml, Image D: Human Tonsil lysate at primary Ab concentration 0.3 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Images A, B, C: Daudi, Jurkat, MOLT-4 cell lysates at primary Ab concentration 0.1 ug/ml (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

3.8 µg/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

SEPT6

NCBI Gene ID

23157