Goat anti-RHBG (mouse) Antibody

• Catalog Number: orb20658
• Category: Antibodies
• Description: Goat polyclonal to RHBG
• Target: RHBG (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PLRGGESDTR
• RRID: AB_10772230
• MW: 49.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 55kDa band observed in Mouse Kidney lysates and in lysates of cell lines NIH3T3 and NSO (calculated MW of 49.5kDa according to NP_067350.2). Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RHBG antibody, anti Rh family, B glycoprotein
• Note: For research use only

orb20658 (1ug/ml) staining of NSO lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.