Goat Rabbit IgG (H&L) Antibody (biotin)

SKU: orb1236938

Description

Goat Rabbit IgG (H&L) Antibody (biotin)

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Reactivity: Rabbit
• Application Notes:
  Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).

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Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Isotype: IgG
• Immunogen: Rabbit IgG - whole molecule
• Purification: Biotin Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only