PIR51/RAD51AP1 Antibody

• Catalog Number: orb18781
• Category: Antibodies
• Description: Goat polyclonal antibody to Rad51
• Target: PIR51 / RAD51AP1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ARVKPLHPNATST
• RRID: AB_10754613
• MW: 38.5; 36.8
• Tested applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:32000, WB: 0.2ug/ml, IHC-P: 4-6µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DNA repair protein RAD51 homolog 1 antibody,
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 10635

Immunohistochemical staining of Human Testis using Rad51 antibody