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Item 1 of 3
Goat anti-MURF1 / TRIM63 (N Term) Antibody
Catalog Number: orb18450
| Catalog Number | orb18450 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to TRIM63 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, IHC |
| Reactivity | Bovine, Canine, Human |
| Dilution range | ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1.25 μg/ml |
| Conjugation | Unconjugated |
| MW | 40.2 |
| Target | MURF1 / TRIM63 (N Term) |
| Entrez | 84676 |
| Protein Sequence | DYKSSLIQDGNPM |
| RRID | AB_10767012 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti TRIM63 antibody, anti MURF1 antibody, anti MU Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.WB: Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Expiration Date | 12 months from date of receipt. |
1.25 μg/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
