MURF1/TRIM63 (N Term) Antibody

SKU: orb18450

Description

Goat polyclonal antibody to TRIM63

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1.25 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.WB: Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MURF1 / TRIM63 (N Term)
• Molecular Weight: 40.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti TRIM63 antibody, anti MURF1 antibody, anti MURF-1 antibody, anti RNF28 antibody, anti SMRZ antibody, anti MURF2 antibody, anti FLJ32380 antibody, anti striated muscle RING zinc finger protein antibody, anti tripartite motif-containing 63 antibody

1.25 μg/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

MURF1 / TRIM63 (N Term)

NCBI Gene ID

84676