MMP12 Antibody

SKU: orb20497

Description

Goat polyclonal antibody to MMP12

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 55kDa band observed in Human Breast Cancer lysates (calculated MW of 54.0kDa according to NP_002417.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MMP12
• Protein Sequence: RPKTSVNLISS
• Molecular Weight: 54
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HME antibody, anti macrophage elastase antibody, anti macrophage metalloelastase antibody, anti matrix metallopeptidase 12 (macrophage elastase) antibody, anti matrix metalloproteinase 12 antibody, anti matrix metalloproteinase 12 (macrophage elastase) antibody, anti MGC138506 antibody, anti MME antibody, anti MMP12 antibody

orb20497 (0.5ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

MMP12

NCBI Gene ID

4321