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MMP12 Rabbit Polyclonal Antibody

Catalog Number: orb13585

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb13585
CategoryAntibodies
DescriptionMMP12 Rabbit Polyclonal Antibody
TargetMMP12
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human MMP12 (201-300/477aa)
UniProt IDP39900
RRIDAB_10751410
MW42/52 kDa
Tested applicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesmatrix metalloproteinase 12; MMP12; EC 3.4.24.65;
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NoteFor research use only
MMP12 Rabbit Polyclonal Antibody

25 ug total protein per lane of various lysates probed with MMP12 polyclonal antibody, unconjugated (orb13585) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP12) Polyclonal Antibody, Unconjugated (orb13585) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP12 Polyclonal Antibody, Unconjugated (orb13585) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP12 Polyclonal Antibody, Unconjugated (orb13585) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP12 Polyclonal Antibody, Unconjugated (orb13585) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP12 Polyclonal Antibody, Unconjugated (orb13585) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

MMP12 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MMP12 Polyclonal Antibody, Unconjugated (orb13585) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

  • MMP12 Antibody (C-term) [orb1930191]

    IF,  IHC-P,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Anti-Mmp12 Rabbit Polyclonal Antibody [orb2569353]

    WB

    Human

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MMP12 Rabbit Polyclonal Antibody [orb2569352]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MMP12 Rabbit Polyclonal Antibody [orb2569354]

    IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Anti-MMP12 Rabbit Polyclonal Antibody [orb2569355]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg