HMHA1/HA-1 Antibody

SKU: orb18670

Description

Goat polyclonal to HMHA1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:32000, WB: 1ug/ml
• Predicted Reactivity: Human
• Application Notes:
  To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 65-70kDa band in Jurkat lysate at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 131kDda according to NP_036424.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HMHA1 / HA-1
• Protein Sequence: TLGSCRERQPEFV
• Molecular Weight: 125
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HA-1 antibody, anti minor histocompatibility antigen HA-1 antibody, anti HLA-HA1 antibody, anti KIAA0223 antibody, anti HMHA1 antibody, anti histocompatibility (minor) HA-1 antibody

Quick Database Links

Gene Symbol

HMHA1 / HA-1

NCBI Gene ID

23526