Histamine Receptor H2 Antibody

• Catalog Number: orb19064
• Category: Antibodies
• Description: Goat polyclonal antibody to HRH2
• Target: Histamine Receptor H2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QEEKPLKLQVWSGTE
• RRID: AB_10753393
• MW: 44.5; 46.9
• Tested applications: ELISA, FC, IF, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to Human NP_001354640.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HRH2 antibody, anti histamine receptor H2 ant
• Note: For research use only
• Entrez: 3274

References

Zhuang, Qian-Xing et al. Histamine Excites Striatal Dopamine D1 and D2 Receptor-Expressing Neurons via Postsynaptic H1 and H2 Receptors Mol Neurobiol, 55, 8059-8070 (2018)

PubMed: 29498008

Reactivity

Rat

2 µg/ml staining of Human Tonsil lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.