Goat anti-Histamine Receptor H2 Antibody

• Catalog Number: orb19064
• Category: Antibodies
• Description: Goat polyclonal antibody to HRH2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 44.5; 46.9
• Target: Histamine Receptor H2
• Entrez: 3274
• Protein Sequence: QEEKPLKLQVWSGTE
• RRID: AB_10753393
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HRH2 antibody, anti histamine receptor H2 ant
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Preliminary experiments in paraffin embedded tonsil, shows strongest staining in groups of cells (probably plasma cells) located in proximity of tonsillar epithelium and in rare cells located either in germinal centres or in the interfollicular area. MicWB: Preliminary experiments gave bands at approx 55kDa and a stronger 28kDa in Human Colon and Duodenum lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 40.1kDa according to NP_071640.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

References

Zhuang, Qian-Xing et al. Histamine Excites Striatal Dopamine D1 and D2 Receptor-Expressing Neurons via Postsynaptic H1 and H2 Receptors Mol Neurobiol, 55, 8059-8070 (2018)

PubMed: 29498008

Reactivity

Rat

Western blot analysis of Human Tonsil lysate using HRH2 antibody

Immunofluorescence analysis of HeLa cells of HRH2 antibody

Flow cytometric analysis of HeLa cells using HRH2 antibody.