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Catalog Number | orb125163 |
---|---|
Category | Antibodies |
Description | Goat Polyclonal to ERO1L. NP_055399.1, PMID: 20834232 and GeneID: 30001. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 54.4 |
Target | ERO1-like (aa105-118) |
Entrez | 30001 |
Protein Sequence | QSDEVPDGIKSASY |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ERO1L antibody, anti ERO1-like (S. cerevisiae Read more... |
Note | For research use only |
Application notes | WB: Approx 70kDa band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kDa according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was. IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of A431 lysate using ERO1L antibody