ERO1-like (aa105-118) Antibody

• Catalog Number: orb125163
• Category: Antibodies
• Description: Goat Polyclonal to ERO1L. NP_055399.1, PMID: 20834232 and GeneID: 30001.
• Target: ERO1-like (aa105-118)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QSDEVPDGIKSASY
• MW: 54.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 70kDa band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kDa according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was. IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ERO1L antibody, anti ERO1-like (S. cerevisiae
• Note: For research use only
• Entrez: 30001

Western blot analysis of A431 lysate using ERO1L antibody