Goat anti-DLG4 / PSD95 Antibody

• Catalog Number: orb20264
• Category: Antibodies
• Description: Goat polyclonal antibody to DLG4.
• Target: DLG4 / PSD95
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EEQARKAFDRATK
• RRID: AB_10749409
• MW: 85.4; 80.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Application notes: This antibody is expected to recognize both reported isoforms (NP_001356.1; NP_001122299.1)ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 110kDa band observed in Mouse Brain lysates (calculated MW of 85.4kDa according to Human NP_001356.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Chaudhury et al, , Am J Physiol Gastrointest Liver Physiol. 2009 Aug 13. PMID: 19679819). Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti discs, large homolog 4 (Drosophila) antibody,
• Note: For research use only
• Entrez: 1742

orb20264 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.