CDC48/YDL126C Antibody

SKU: orb195329

Description

Goat polyclonal antibody to CDC48

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat, Yeast
• Application Notes:
  WB: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03µg/ml.. IHC: Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CDC48 / YDL126C
• Protein Sequence: HTKNMKLADDVDLE
• Molecular Weight: 92
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CDC48 antibody, anti CDC48p antibody, anti YDL126C antibody, anti VCP antibody, anti transitional endoplasmic reticulum ATPase antibody, anti valosin containing protein antibody, anti p97 antibody, anti TERA antibody

Western blot analysis of HeLa lysate using CDC48 antibody

Quick Database Links

Gene Symbol

CDC48 / YDL126C

NCBI Gene ID

7415