CDC48/YDL126C Antibody

SKU: orb1248155

Description

CDC48/YDL126C Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat, Yeast
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03µg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HTKNMKLADDVDLE
• Target: CDC48 / YDL126C
• Molecular Weight: Approx 100kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA

orb1248155 (0.01 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CDC48 / YDL126C

UniProt

P55072

RefSeq (Protein):NP_010157.1