CD74 (aa159-171) Antibody

• Catalog Number: orb195359
• Category: Antibodies
• Description: Goat polyclonal antibody to CD74
• Target: CD74 (aa159-171)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KGSFPENLRHLKN
• MW: 33.5; 26.4
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: This antibody was observed to recognize variations of bands at approx. 26kDa, 30kDa,35kDa and 70kDa in Human Duodenum and Lung lysates (calculated MW of 26.4kDa according to NP_004346.1 and 33.5kDa according to NP_001020330.1. All bands were blocked by incubation with the immunizing peptide and have been observed by other commercial sources. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml Flow Cytometry: Flow cytometric analysis of Caco--2 cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD74 antibody, anti DHLAG antibody, anti HLAD
• Note: For research use only
• Entrez: 972
• Expiration Date: 12 months from date of receipt.

Primary incubation 1 hour at room temperature. Images A, B, C: Human Duodenum lysate, Human Lung lysate 1 and Human Lung lysate 2 at primary Ab concentration 1 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Flow cytometric analysis of paraformaldehyde fixed CaCo2 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.