ATG4D Antibody

SKU: orb20182

Description

Goat polyclonal antibody to ATG4D

Research Area

Apoptotic, Autophagic, Cancer, Cardiovascular Disease, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ATG4D
• Protein Sequence: CSQLAQPTLRLPRT
• Molecular Weight: 52.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG4D antibody, anti ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody, anti APG4-D antibody, anti APG4D antibody, anti AUTL4 antibody, anti APG4 autophagy 4 homolog D antibody, anti AUT-like 4, cysteine endopeptidase antibody, anti autophagin-4 antibody, anti autophagy-related cysteine endopeptidase 4 antibody, anti cysteine protease involved in autophagy antibody

Quick Database Links

Gene Symbol

ATG4D

NCBI Gene ID

84971