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Catalog Number | orb1256997 |
---|---|
Category | Antibodies |
Description | GNL3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 62kDa |
Target | GNL3 |
UniProt ID | Q9BVP2 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | GNL3, guanine nucleotide binding protein-like 3 (n Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:10 - 1:100IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of 293T cells, using GNL3 antibody (orb1256997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using GNL3 antibody (orb1256997). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug GNL3 antibody (orb1256997). Western blot was performed from the immunoprecipitate using GNL3 antibody (orb1256997) at a dilution of 1:500.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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