GNB3 Antibody

• Catalog Number: orb1539162
• Category: Antibodies
• Description: GNB3 Antibody
• Target: GNB3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence SGHDNRVSCLGVT, from the internal region of the protein sequence according to NP_002066.1, NP_067642.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 35kD band observed in lysates of cell line HepG2 (calculated MW of 37.2kD according to NP_002066.1. Recommended concentration: 0.1-0.3 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GNB3, G protein, beta-3 subunit, Transducin beta c
• Note: For research use only

GNB3 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.