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GNB3 Antibody

SKU: orb1539162

Description

GNB3 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution rangeIHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
ReactivityBovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 35kD band observed in lysates of cell line HepG2 (calculated MW of 37.2kD according to NP_002066.1. Recommended concentration: 0.1-0.3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence SGHDNRVSCLGVT, from the internal region of the protein sequence according to NP_002066.1, NP_067642.1.
TargetGNB3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

GNB3, G protein, beta-3 subunit, Transducin beta chain 3

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

GNB3 Antibody

GNB3 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GNB3 Antibody (orb1539162)

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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