GNAQ Antibody

SKU: orb639685

Description

GNAQ is 359 amino acids long and is identical in all but 1 amino acid residue to the mouse protein. Analysis of human genomic DNA revealed an intronless sequence with strong homology to human GNAQ cDNA. In comparison to GNAQ cDNA, this genomic DNA sequence included several small deletions and insertions that altered the reading frame, multiple single based changes, and a premature termination codon in the open reading frame, all hallmarks of a processed pseudogene. Probes derived from human GNAQ cDNA sequence mapped both chromosomes 2 and 9 in higher constringency genomic blot analyses of DNA from a panel of human/rodent hybrid cell lines.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA: order BSA-free format for coating,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the GNAQ antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: GNAQ/2434
• Immunogen: A recombinant human full length protein was used as the immunogen for the GNAQ antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free GNAQ antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

P50148