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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human FGF basic, Tag Free at 0.5 ug/mL (100 uL/well) can bind Mouse Glypican 3, His Tag with a linear range of 1-16 ng/mL. |
| Target | Glypican 3 |
| Tag | His Tag |
| MW | 38.0 kDa and 24.4 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Gln 25 - Met 557 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Immobilized Human FGF basic, Tag Free at 0.5 ug/ml (100 uL/well) can bind Mouse Glypican 3, His Tag with a linear range of 1-16 ng/mL.
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Protocol Information
Glypican 3 Recombinant Protein (orb1227682)
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