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Description
Research Area
Images & Validation
−| Tested Applications | ICC, IP |
|---|---|
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Clone No. | ROF7 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Rab1-GTP is detected by immunocytochemistry using anti-Rab1-GTP, mAb (ROF7). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab1-GTP, mAb (ROF7) (1mg /ml) in PBS-BSA-Saponin). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
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UniProt Details
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Protocol Information
Functional Rab1-GTP Antibody, mAb (recombinant) (orb1788500)
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