FOXD1 Antibody (N-term)

SKU: orb32533

Description

Peptide affinity purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:2000, IHC-P - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD56520
• Immunogen: Synthetic Peptide
• Target: FOXD1
• Molecular Weight: 46140
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Forkhead box protein D1 antibody, anti-FOXD1 antibody, anti-FKHL8 antibody, anti-FREAC4 antibody

FOXD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FOXD1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

All lanes: Anti-FOXD1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: Y79 whole cell lysate. Lane 5: Mouse kidney tissue lysate. Lane 6: Mouse eyeball tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

FOXD1

UniProt

Q16676

RefSeq (Protein):NP_004463.1

Zhanwang Wang 1, Yi Jin 2, Dong He 3, Yuxing Zhu 1, Mengqing Xiao 1, Xiaoming Liu 4, Yaxin Cheng 1, Ke Cao Targeting ALG3/FOXD1/BNIP3 Axis Prevents Mitophagy and Gemcitabine Resistance of Nasopharyngeal Carcinoma Int J Biol Sci ., (2025)

PubMed: 40083705

Applications

IHC

Reactivity

Human