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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Folic acid-BSA conjugate at 20 ug/mL (100 uL/well) can bind Mouse FOLR1, His Tag with a linear range of 0.078-1.25 ug/mL. |
| Target | FOLR1 |
| Tag | His Tag |
| MW | 26.2 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Thr 25 - Ser 232 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Mouse FOLR1 Protein [orb2994754]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 25.3 KDa. Observed: 33-37 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FOLR1 Protein [orb2994909]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 25.7 KDa. Observed: 28-38 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FOLR1 Protein (Biotin) [orb2992834]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 53.4 KDa. Observed: 61-73 KDa, reducing conditions
20 μg, 100 μgRecombinant Rhesus Macaque FOLR1 Protein [orb2993002]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 25.5 kDa. Observed: 28-38 kDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FOLR1 Protein [orb2993003]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 51.6 kDa. Observed: 55-75 kDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μg

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Immobilized Folic acid-BSA conjugate at 20 ug/ml (100 uL/well) can bind Mouse FOLR1, His Tag with a linear range of 0.078-1.25 ug/ml.
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Protocol Information
FOLR1 Recombinant Protein (orb1227686)
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