FGFR1 Antibody

• Catalog Number: orb1263060
• Category: Antibodies
• Description: FGFR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This FGFR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the N-terminal region of human FGFR1.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 92 kDa
• Target: FGFR1
• UniProt ID: P11362
• NCBI: P11362
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Fibroblast growth factor receptor 1, FGFR-1, Basic
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of MCF-7 cells using FGFR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antibody is used in Western blot to detect FGFR1 in NIH-3T3 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i, iv, vii), A1B3 is visualized as green fluorescence (ii, v, viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii, vi, ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25.