AHSG Antibody

SKU: orb344218

Description

Fetuin antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:40.000 - 1:200,000, WB: 1:10,000 - 1:20,000
• Reactivity: Human
• Application Notes:
  This purified polyclonal antibody has been tested by western blot. Although not tested, this antibody is likely functional in ELISA, immunohistochemistry, and immunoprecipitation. A doublet band or slightly lower molecular weight band (see image) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
• Target: AHSG
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Fetuin antibody, 59 kDa bone sialic acid-containing protein antibody, A2HS antibody, Aa2-066 antibody, AHS antibody, Ahsg alpha-2-HS-glycoprotein antibody, AHSG antibody, Alpha 2 Z globulin antibody, Alpha-2-HS-glycoprotein antibody, Ba-alpha-2-glycoprotein, Fetuin-A, PRO2743

AHSG Antibody

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 µl of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10000 and 1:20000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5000 dilution of IRDye™800 conjugated Donkey anti-Goat IgG for 45 min at room temperature.

Quick Database Links

Gene Symbol

AHSG

UniProt

P02765

RefSeq (Protein):NP_001613.2