FCGR1A/CD64 Rabbit Polyclonal Antibody

• Catalog Number: orb1816889
• Category: Antibodies
• Description: FCGR1A/CD64 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse FCGR1A/CD64 (131-230/404aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: FCGR1A
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: High affinity immunoglobulin gamma Fc receptor I;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-CD64 antibody (orb1816889), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat Anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Lung tissue lysates, Primary: Anti-FCGR1A/CD64 (orb1816889) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 62 kDa.