FAM-Phe-DAP Green FLISP Assay Kit

SKU: orb1409921

Description

Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The DAP group phosphonylates the active site serine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.

Images & Validation

• Application Notes:
  1. Add 1 part Antigen-Down HRP Conjugate Stabilizer to 4 parts deionized water and mix for 5-15 minutes., 2. Add the anti-IgG-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes., 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C and protected from light, providing a useful shelf-life of 6-12 months.

Key Properties

• Sample Types: Cell culture, tissue

Storage & Handling

• Storage: FLISP at ≤ -20°C, other components at 2-8°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only