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F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated

F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated

Catalog Number: orb346781

DispatchUsually dispatched within 5-10 working days
$ 510.00
Catalog Numberorb346781
CategoryAntibodies
DescriptionF(ab')2 Mouse IgG (H&L) antibody (FITC)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG F(ab')2
ConjugationFITC
ReactivityMouse
Form/AppearanceLyophilized
Concentration10.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
ImmunogenMouse IgG whole molecule
Tested applicationsFC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat F(ab')2 anti-Mouse IgG Antibody fluorescein C
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NoteFor research use only