F(ab')2 Human IgA (alpha chain) Antibody Alkaline Phosphatase Conjugated

SKU: orb420161

Description

F(ab')2 Human IgA (alpha chain) antibody (AP)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
• Reactivity: Human
• Application Notes:
  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Human IgA alpha heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted powder. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 15 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 0.7 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 anti-Human IgA (alpha chain) Antibody alkaline phosphatase Conjugation, Goat F(ab')2 anti-Human IgA alpha alk phos Conjugated Antibody