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Catalog Number | orb346759 |
---|---|
Category | Antibodies |
Description | F(ab')2 Guinea Pig IgG F(ab')2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Guinea pig |
Isotype | IgG F(ab')2 |
Immunogen | Guinea Pig IgG F(ab')2 fragment |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin. |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | rabbit F(ab')2 Anti-GUINEA PIG IgG F(ab')2 fragmen Read more... |
Note | For research use only |
Application notes | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. |
Expiration Date | 12 months from date of receipt. |
TRITC | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig IgG, anti-Guinea Pig IgG F(ab’)2 and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(c) or anti-Pepsin. | |
Guinea Pig |
IP | |
Agarose | |
This product is an purified Guinea Pig IgG isolated from normal Guinea Pig Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Guinea Pig IgG. | |
Guinea Pig |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig IgG, anti-Guinea Pig IgG F(ab’)2 and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(c) or anti-Pepsin. | |
Guinea Pig |
DOT | |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(ab’)2 and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(c) or anti-Pepsin. | |
Guinea Pig |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(ab’)2 and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(c) or anti-Pepsin. | |
Guinea Pig |
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