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Catalog Number | orb346750 |
---|---|
Category | Antibodies |
Description | F(ab')2 Dog IgG F(ab')2 antibody (FITC) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, FLISA, IF |
Reactivity | Canine |
Isotype | IgG F(ab')2 |
Immunogen | Dog IgG F(ab')2 fragment |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000 |
Form/Appearance | Lyophilized |
Purity | This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab’)2 and Dog Serum. No reaction was observed against Dog IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin. |
Conjugation | FITC |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | Rabbit F(ab')2 anti-Dog IgG F(ab')2 Antibody Fluor Read more... |
Note | For research use only |
Application notes | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Expiration Date | 12 months from date of receipt. |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Pepsin. | |
Dog |
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