F(Ab')2 Biotin Antibody Phycoerythrin Conjugated

SKU: orb345927

Description

F(Ab')2 antibody (RPE)

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:100-1:250, FC: 1 µg/1,000,000 cells, WB: 1:100-1:250
• Application Notes:
  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum and Biotinylated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Biotin Phycoerythrin Conjugated Antibody, Goat F(ab')2 Anti Biotin PE Conjugated Antibody, Goat Fab2 Anti-Biotin Antibody Phycoerythrin Conjugation