Fab Human IgG (H&L) Antibody

• Catalog Number: orb348465
• Category: Antibodies
• Description: Fab Human IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG Fab
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
• Immunogen: Human IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:30,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Fab Anti-Human IgG (H&L) Antibody has been tested by SDS-PAGE and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit Fab Anti-Human IgG Antibody, Rabbit Fab Fra
• Note: For research use only

Fluorescence microscopy using Rabbit Fab Anti-Human IgG. Analysis of human IgGs displayed on the surface of OCMS™ hybridomas. (a) 8C5 OCMS™ (top row) and B5-6T OCMS™ (bottom row) cells were incubated overnight with the RAH (AB14), followed by biotinylated rabies GP and Alexa Fluor 488® streptavidin (green) (AB3), and visualized by fluorescence microscopy. Images are shown with DAPI (blue) (right) or without DAPI (left). Scale bar = 5 µm. (b) The 4G4 OCMS™ (top row) and 8C5 OCMS™ (bottom row) hybridomas were incubated overnight with a rabbit Fab anti-human IgG (AB17), then for an hour with additional rabbit Fab anti-human IgG and Sabin type III PV (left column) or GP (right column), followed by an Alexa Fluor® 488 anti-human IgG (green) (AB2). Nuclei were stained with DAPI (blue). Scale bar = 10 µm. (c) In this mixing experiment, non-secretor OCMS™ cell lines were labeled with CFSE (green) and mixed in a 10:1 ratio with IgG-secreting OCMS™ hybridomas. Cells were incubated overnight with rabbit F(ab')2 anti-human IgG (AB15) then washed and incubated with biotinylated Protein A (AB12) and APC streptavidin (red) (AB4). (Top row) 8C5 OCMS™ vs. B5-6T OCMS™ CFSE. (Bottom row) 4G4 OCMS™ vs. LCX OCMS™ CFSE. With DAPI (blue) (right column) or without (left column). Scale bar = 5 µm.