FBXO9 Antibody

SKU: orb345535

Description

F-Box Only Protein 9 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Although the predicted MW of FBOX9 isoform 1 is 52 kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa. This band is believed to be FBOX9 and the higher apparent MW may be due to its association with other proteins. Isoforms 1, 2 and 3 have reported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.
• Target: FBXO9
• Purity: This affinity-purified antibody is directed against human FBOX9 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) as well as chicken (88%) and Xenopus laevis (82%). Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.53 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-F-Box Only Protein 9 antibody, rabbit anti-FBXO9 antibody, FBX9, VCIA1, Cross-immune reaction antigen 1 antibody, Renal carcinoma antigen NY-REN-57 antibody

Western blot using Biorbyt's affinity purified anti-FBOX9 antibody shows detection of a band at ~100 kDa (arrowhead) believed to correspond to FBOX9 present in a MCF7 whole cell lysate (p/n orb348664). Specific band reactivity is greatly diminished when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).

Quick Database Links

Gene Symbol

FBXO9

UniProt

Q9UK97

RefSeq:53692184