F-Box Only Protein 9 antibody

• Catalog Number: orb345535
• Category: Antibodies
• Description: F-Box Only Protein 9 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.
• Concentration: 1.53 mg/mL
• Dilution range: ELISA: User Optimized, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human FBOX9 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) as well as chicken (88%) and Xenopus laevis (82%). Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9UK97
• NCBI: 53692184
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-F-Box Only Protein 9 antibody, rabbit
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Although the predicted MW of FBOX9 isoform 1 is 52 kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa. This band is believed to be FBOX9 and the higher apparent MW may be due to its association with other proteins. Isoforms 1, 2 and 3 have reported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCF7 whole cell lysate using F-Box Only Protein 9 antibody