EZH1 Antibody

SKU: orb420354

Description

Ezh1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000, WB: 1.0 ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Tested using positive control NIH/3T3 nuclear extract p/n W10-001-A74.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
• Purity: Anti-EZH1 is directed against human EZH1 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.95 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1

Western Blot of Rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder. Lane 1: NIH/3T3 WCL (p/n orb348714). Lane 2: NIH/3T3 nuclear extract (p/n orb348724). Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 60 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in lane 2 NIH/3T3 nuclear extract (p/n orb348724).

Quick Database Links

UniProt

Q92800

RefSeq (Protein):NP_001982.2