ExoS | Exoenzyme S Antibody

SKU: orb303180

Description

Chicken polyclonal antibody to ExoS

Research Area

Microbiology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1: 5000
• Reactivity: Bacteria
• Application Notes:
  Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Key Properties

• Host: Gallus
• Clonality: Polyclonal
• Immunogen: amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion, Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel, Gel piece has been used for immunizations,
• Molecular Weight: 48 kDa
• Purity: Total IgY
• Purification: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at 4°C; make aliquots to avoid working with a stock, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
• Form/Appearance: Liquid in PBS pH 8,0, 0,02% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q51449