ETV1 Rabbit Polyclonal Antibody

SKU: orb329990

Description

Rabbit polyclonal antibody to ETV1

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ETV1
• Target: ETV1
• Molecular Weight: 55kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti DKFZp781L0674 antibody, anti ER81 antibody, anti MGC104699 antibody, anti MGC120533 antibody, anti MGC120534 antibody

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Several isoforms contain this peptide sequence from 43 kDa to 57 kDa and the protein may be phosphorylated and/or sumoylated.

Sample Tissue: Human MDA-MB-435s, Antibody Dilution: 1.0 ug/mL.

Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5 ug/mL using anti-ETV1 antibody (orb329990).

WB Suggested Anti-ETV1 Antibody, Positive Control: Lane 1: 100 ug untransfected HEK293 lysate Lane 2: 33 ug ER81 transfected HEK293 lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit-HRP, Secondry Antibody Dilution: 1:2000.

WB Suggested Anti-ETV1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:1562500, Positive Control: HT1080 cell lysate.

Quick Database Links

Gene Symbol

ETV1

UniProt

P50549

RefSeq (Protein):NP_004947