ETFB Antibody

• Catalog Number: orb1248901
• Category: Antibodies
• Description: ETFB Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-QVTLEGDKLKVERE
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ETFB
• UniProt ID: P38117
• NCBI: NP_001976.1, NP_001014763.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ETFB, electron-transfer-flavoprotein, beta polypep
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 27kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kDa according to NP_001976.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248901 (0.1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248901 (3.8 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.