Estrogen Receptor beta 1 Antibody

• Catalog Number: orb699734
• Category: Antibodies
• Description: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids CEDSKSKEGSQNPQSQ from the C terminus of the human estrogen receptor beta 1 isomer were used as the immunogen for the Estrogen Receptor beta 1 antibody.
• UniProt ID: Q92731
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the Estrogen Receptor beta 1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PGP5/10
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the Estrogen Receptor beta 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Estrogen Receptor beta 1 antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 1 antibody (clone PGP5/10); Red=isotype control, Blue= Estrogen Receptor beta 1 antibody.

IHC staining of FFPE human breast carcinoma with Estrogen Receptor beta 1 antibody (clone PGP5/10). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 1 antibody (clone PGP5/10) as confirmation of integrity and purity.